Full text
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| Author(s): |
Ana Débora Nunes Pinheiro
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2015-03-02 |
| Examining board members: |
Cristiane Masetto de Gaitani
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| Advisor: | Cristiane Masetto de Gaitani |
| Abstract | |
Nebivolol is an anti-hypertensive drug, commercialized as a racemic mixture of RSSS and SRRR-nebivolol. Both enantiomers have distintict pharmacological properties, what suggests a different pharmacokinectis between them. Therefore, it was developed two methods for the nebivolol analysis by capillary electropforesis (CE), one of them is achiral and the other is chiral. The first method aimed the analysis of tablets. The analytical conditions were determined: silica fused capillary 50 ?m internal diameter (i.d.) and 38 cm effective length (ef. l.), running electrolyte composed by 50 mM sodium acetate buffer, pH 4.0, 30 °C temperature, 0 kV applied voltage and 200 nm UV detection. The developed method allowed a quickly and efficient tablets analysis, being simple, selective, accurate and precise, and it is also in accordance with ANVISA (2003) analytical methods validation guide, and it was applied to the quantification of nebivolol in tablets. The second method aimmed to analyze the enantiosselective nebivolol binding to HSA. After the evaluation of many parameters, it was stabilished the following analytical conditions: 50 ?m i.d. and 38 cm ef.l. fused silica capillary, running electrolyte composed by 50 mM sodium acetate buffer, 12.5 mM carboxymethyl- ?-cyclodextrin, acetonytrile 1%, pH 4.0, 25 oC, 25 kV applied voltage and 200 nm UV detection; and as stacking technique it was applied field amplified sample injection with water plug(5 psi, 5 s) and 5 kV por 30 s eletrokinect injection. At this condition, it was possible to achive 1.58 resolution and less than 25 minutes of analysis. At the HSA binding study, it was observed an enantiosselectivity on the binding; however this study still needs better desing in conserne to analyte and protein concentration, as well as, incubation time and filtration proceadure to the separion of binded and free fractions. (AU) | |
| FAPESP's process: | 12/16597-3 - Evaluation of capillary electrophoresis to quantificate nebivolol on solid pharmaceutical formulation and on enantioselective binding to human plasma proteins. |
| Grantee: | Ana Débora Nunes Pinheiro |
| Support Opportunities: | Scholarships in Brazil - Master |