Evaluation of xylose catabolism genes and their potential for the generation of bioproducts.

Author(s)

Juliano Cherix

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-04-06

Examining board members

Luiziana Ferreira da Silva; Regina Lúcia Baldini; Andrea Balan Fernandes

• Advisor: Luiziana Ferreira da Silva

Abstract

Xylose is a major component of lignocellulosic materials, which are of great interest for the production of bio-products, such as ethanol and polyhydroxyalkanoates (PHA). To improve the consumption of xylose in Burkholderia sacchari, a major PHA producer, the following genes, encoding xylose isomerase, were introduced in these bacteria: xylABs, xylABc, xylAPl, xylABp and xylABx, respectively from B. sacchari, B. cenocepacia, Photorhabdus luminescens, B. phymatum e B. xenovorans. The gene of B. sacchari (xylA*) was also synthesized with several modifications described in the literature as able to increase the consumption of xylose in other organisms. Recombinant strains harboring B. sacchari xylABs and xylA* gene had an increase of approximately 30% in the xylose consumption compared to the control strain, and those harboring xylABx and xylABp gene an increase of 23%. These four recombinant strains were those that were able to produce more P3HB, approximately 70% more than the control strain. (AU)

• FAPESP's process: 12/11529-0 - The evaluation of genes for the xylose catabolism and their potential for bioproducts
• Grantee: Juliano Cherix
• Support Opportunities: Scholarships in Brazil - Master