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Isolation of microsatelites and genetic analysis of Spix\'s macaw (Cyanopsitta spixii, Psitaciformes, Aves)

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Rafaella Sávia Monteiro
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB)
Defense date:
Examining board members:
Cristina Yumi Miyaki; Maria Cristina Arias; Mercival Roberto Francisco
Advisor: Cristina Yumi Miyaki

Spix\'s macaw (Cyanopsitta spixii) is one of the most endangered birds of the world and is extinct in the wild. Several coordinated studies are being conducted for its management and conservation in captivity for future reintroduction. Microsatellites are useful markers to estimate relatedness between individuals. This information can be used to minimize the deleterious effects of inbreeding and loss of genetic diversity of captive birds, recommending less closely related pairs for the breeding program. The present study aims to identify specific polymorphic microsatellites to assess the levels of genetic variability of the species and the genetic relatedness among birds for the genetic management of the population. The partial genome of an individual was sequenced on a 454 GS FLX (Roche) platform. Twenty-five pairs of primers were designed, being 20 for di- and five tetra-nucleotide microsatellites. Nineteen microsatellites were amplified and tested in 12 selected individuals. Fourteen microsatellites were polymorphic. Heterologous microsatellites were also used in the analyses. Two microsatellites were not in Hardy-Weinberg equilibrium and five presented linkage disequilibrium. Exclusion paternity probability when one parental is known was 94.8% and identity probability was 0.00000793. Mean allele richness was 2.49 alleles per microsatellite, confirming the low genetic diversity of the species. The current captive population has lost some alleles. The mean relatedness among individuals was, at least, similar to the one between first cousins and some founders are very closely related. Based on the relatedness index, some unrelated potential couples can become important for the captive program. (AU)

FAPESP's process: 12/08413-0 - Isolation and genotyping of microsatellites of Spix´s macaw (Cyanopsitta spixii, Psittaciformes, Aves)
Grantee:Rafaella Sávia Monteiro
Support Opportunities: Scholarships in Brazil - Master