Fungal diversity, polyphasic analysis of the genus Fusarium and determination of deoxynivalenol and zearalenone in wheat grains from different regions of Brazil.

Author(s)

Sabina Moser Tralamazza

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-07-01

Examining board members

Benedito Correa; Eliana Badiale Furlong; Kelly Ishida; Ida Chapaval Pimentel; Myrna Sabino

• Advisor: Benedito Correa

Abstract

The present work aimed to use a polyphasic approach, by phenotypic and genotypic characteristics for the identification of Fusarium strains from wheat grains as well to investigate the presence of deoxynivalenol and zearalenone on wheat grains from three wheat producers States (Parana, Rio Grande do Sul and Sao Paulo). Our results showed that the main genera found were Alternaria, Fusarium and Epicoccum. Genotype detection from qPCR revealed predominance of 15-ADON, followed by NIV and 3-ADON. The qPCR demonstrated that 15-ADON genotype was responsible for 96% of all DNA quantified, followed by NIV with 3.84% and 3-ADON with 0.06%, indicating that 15-ADON is the main trichothecene genotype from Brazilian wheat grains. The toxin deoxynivalenol was detected in all 150 samples analyzed from wheat grains, with medians of 323, 466 and 783 µg/kg in SP, PR and RS, respectively. The zearalenone determination showed contamination on 100%, 80% and 42% of wheat grains from RS, PR and SP State and medians of 843, 100 and 14 µg/kg, respectively. (AU)

• FAPESP's process: 11/19470-1 - Poliphasic study for the identification of Fusarium spp deoxynivalenol producing strains
• Grantee: Sabina Moser Tralamazza
• Support Opportunities: Scholarships in Brazil - Doctorate