Full text
| |
| Author(s): |
Berna Inés Giménez Kappeler
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2015-11-10 |
| Examining board members: |
Luiz Lehmann Coutinho;
Andrezza Maria Felicio Ament;
Luciana Correia de Almeida Regitano
|
| Advisor: | Luiz Lehmann Coutinho |
| Abstract | |
Brazil occupies the second world position in beef production and thus, the implementation of new tools to select zebuine animals (Bos indicus) with better beef quality has an important contribution to the competitiveness of beef cattle. Inside this context, to comprehend the microRNAs expression patterns involved in the processes that are related to beef tenderness is essential to the meat production since this organoleptic characteristic has a high value in meat acceptance by the consumers. The advent of new generation sequencing technologies along with the biotechnology tools usage has allowed large-scale genome studies as well as faster and cheaper analysis. In this study, samples of the Longissimus dorsi muscle from 34 animals of Nellore cattle breed with extreme estimated genetic value (EBV) for shear force (FC) were used. The total RNAs were extracted, libraries of microRNA were built and finally the sequencing was performed using the Illumina (MiSeq) platform equipment. Data processing was done using FastQC, Cutadapt and miRDeep2 softwares while the differential expression analyzes were realized through the statistical package QuasiSeq. Using a false discovery rate (FDR) criteria below 0.1, three microRNAs (bta-mir- 182, bta-mir-183, bta-mir-338) were identified as differentially expressed among the group of animals with extreme EBV values for FC. A total of 1024 target genes were predicted and functional analyzes of enrichment were performed using bioinformatics tools. Many metabolic networks and pathways such as the apoptosis signalization and cell regulation mechanisms by calpain protease were obtained, demonstrating therefore that the identified target genes would be involved in many metabolic processes related with the beef tenderness. (AU) | |
| FAPESP's process: | 14/00943-5 - Identification of microRNAs involved in meat tenderness in Nellore cattle |
| Grantee: | Berna Ines Gimenez Kappeler |
| Support Opportunities: | Scholarships in Brazil - Master |