Effects of pinealectomy and blockade of signaling by glucocorticoid receptor on the metabolic and inflammatory liver fuctions.

Author(s)

Sandra Campos Rodrigues

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-11-10

Examining board members

Silvana Auxiliadora Bordin da Silva; Bruno Cogliati; Pedro Augusto Carlos Magno Fernandes; Gisele Akemi Oda; Lício Augusto Velloso

• Advisor: Silvana Auxiliadora Bordin da Silva

Abstract

Circadian biological rhythms guarantee the temporal relationship between living beings and the environment. Metabolic regulation relates to the rhythm to the light-dark cycle, with importance of the actions of melatonin and glucocorticoids in the liver. We investigated the participation of glucocorticoids in the metabolic adjustment mediated by rhythmic secretion of melatonin on the liver inflammatory context. Male Wistar rats pinealectomized treated or not with RU 486 had the circadian levels of circulating corticosterone, insulin and glucose measured along 8ZTs and on ZT10. At this point, we analyzed mRNA and protein expression related to metabolic and inflammatory actions. In vivo tests to glucose and pyruvate tolerance and rating the phosphorylation of Akt in response to insulin in liver at ZT10, with the changes in weight and body composition, glycogen content and liver histology, indicate influence of corticosterone in gluconeogenesis and correlation of both hormones in energy alterations and on the installation of a hepatic pro-inflammatory process. (AU)

• FAPESP's process: 11/12329-1 - ROLE OF GLUCOCORTICOIDS ON CIRCADIAN REGULATION OF LIVER ENERGY METABOLISM.
• Grantee: Sandra Campos Rodrigues
• Support Opportunities: Scholarships in Brazil - Doctorate