Interaction between immature dental pulp stem cells and canine osteosarcoma

Author(s)

Dayane Alcântara

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
• Defense date: 2014-10-31

Examining board members

Maria Angélica Miglino; Mirela Tinucci Costa; Fernanda Faião Flores; Julia Maria Matera; Ana Claudia Oliveira Carreira Nishiyama

• Advisor: Maria Angélica Miglino

Abstract

Osteosarcoma is a malignant bone tumor most frequent in dogs. It has fast growth and high metastatic potential. Thus, the dog is an useful model for the study of human disease, due to the clinical and histological similarities found in both species. Currently, studies about stem cells are promising considering its high therapeutic potential. However, many doubts still exist regarding the treatment of tumors using cell therapy. This theme is little known and studied. Therefore, the aim of this study was to evaluate the interaction between stem cells obtained from canine immature dental pulpstem cells with osteosarcoma cells derived from dogs. Cellular coculture were performed using cells derived from canine dental pulp, canine osteosarcoma and canine normal bone. The morphological aspects of cocultured cells and control were analyzed, as well as proliferative activity, cell death, the mitochondrial membrane electric potential and gene expression. In summary, it was concluded that the interaction between stem cells from canine immature dental pulp and canine osteosarcoma cells did not show morphological changes. However, stem cells derived from canine dental pulp and healthy canine fetal bone serve to support tumor growth. Furthermore, the cell coculture in all groups tested, causes changes in gene and protein expression. (AU)

• FAPESP's process: 11/11982-3 - "Interaction between canine dental pulp stem cells and canine osteosarcoma"
• Grantee: Dayane Alcântara
• Support Opportunities: Scholarships in Brazil - Doctorate