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| Author(s): |
Aline Elisa Santana
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2015-12-07 |
| Examining board members: |
Carlos Eduardo Larsson;
Ana Claudia Balda;
Carlos Pelleschi Taborda
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| Advisor: | Carlos Eduardo Larsson |
| Abstract | |
The number of dogs and cats affected by ringworm seen at teaching hospitals and veterinary clinics in Brazil ranges from 1,7 to 11,5 %. And although the percentile of ringworm cases in veterinary practice routine is reduced when compared with other skin diseases, it has relevance because it is anthropozoonosis with public health significance. In humans, 15% of ringworm's (tinea) cases come from zoonotic transmission and most of these cases are acquired through contact with affected cats. Animal dermatophytosis is a skin disease caused by keratinolytics and keratinophilics fungi belonging to the genera Microsporum and Trichophyton. The zoophilic fungus M. canis is the most frequently isolated dermatophyte in domestic carnivores, being regarded as the causative agent associated with 90 to 100% of ringworm cases. Dermatophytes are transmitted by direct contact with scales or hairs infected by arthrospores, present in the animals, in the environment or in fomites. The differential diagnosis for dermatophytosis should be considered in every case of alopecia, keratotic scales and crusty lesions. The diagnosis is challenging and the use of fungal cultures is quite useful but requires laboratorial experience and need at least 21 days to get the result. Given the scarcity of studies involving alternative diagnostic procedures, like serological techniques for the dermatophytosis's diagnosis, and the need for reliable and rapid tests for early diagnosis, this study aimed to develop and evaluate the diagnostic efficacy of enzyme immunoassays ELISA (Enzyme Linked Immuno Sorbent Assay) and Western blot (WB), for the diagnosis of feline's dermatophytosis caused by M. canis in Persian cats. Seventy cats of various ages and any of the sexes were divided into three groups: S (symptomatic) with 20 animals, AS (asymptomatic) with 30 animals and N (negative) with other 20 cats. All animals were submitted to mycological cultivation, Wood lamp and blood samples for carrying out the serological tests. Comparing the ELISA values between positive and negative groups yielded 94% sensitivity and 75% specificity. There was no statistic difference between groups symptomatic and asymptomatic (p <0,05). The WB technique detected 13 bands of the following weights: 132, 83, 75, 73, 57, 54, 50, 40, 35, 33, 32, 30 and 25 kDa. The 50 kDa protein was considered the most immunogenic protein, observing reactivity in 83.3% in the symptomatic group and 66.6% in the asymptomatic group. The study concludes that the ringworm induces clear humoral immune response. In addition, the ELISA and WB techniques are effective for the diagnosis of dermatophytosis caused by M. canis in symptomatic and asymptomatic Persian cats (AU) | |
| FAPESP's process: | 13/18243-7 - Development of enzyme immunoassays (ELISA and Western Blot) for the serological diagnosis of feline dermatophytosis caused by Microsporum canis |
| Grantee: | Aline Elisa Santana |
| Support Opportunities: | Scholarships in Brazil - Master |