Analysis of the participation of Oligopeptidase B and Cytoplasmic Tryparedoxin Peroxidase in virulence of Leishmania (Leishmania) amazonensis.

Author(s)

Karoline Mathias Leite

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-12-15

Examining board members

Beatriz Simonsen Stolf Carboni; Andréa Cristina Fogaça; Luiz Ricardo Orsini Tosi

• Advisor: Beatriz Simonsen Stolf Carboni

Abstract

The survivability of Leishmania within specialized cells in the destruction of pathogens due to the parasite\'s ability to circumvent the microbicidal property for the production of molecules called virulence factors. Among the proteins differentially expressed in a previous study from our laboratory, we found isoforms of OPB, a peptidase serine and CPX, antioxidant protein. Indeed, promastigotes of L. (L.) Major disabled in OPB showed a significant reduction in infection and survival in macrophages in vitro and slower evolution of lesions in a murine model of infection in the leg. Similarly, promastigotes of L. (L.) Donovani overexpressors CPX showed higher parasite load in macrophages in vitro. Given this information and the importance of L. (L.) amazonensis in the epidemiology of leishmaniasis in Brazil, our goal is to analyze the importance of OPB and CPX virulence of this species using overexpressors parasites and soluble proteins in murine models of infection in vitro and in alive. (AU)

• FAPESP's process: 13/13527-7 - Analysis of the participation of oligopeptidase B and cytoplasmic tryparedoxin peroxidase in virulence of Leishmania (Leishmania) amazonensis
• Grantee: Karoline Mathias Leite
• Support Opportunities: Scholarships in Brazil - Master