Full text
| |
| Author(s): |
Lennon Ramos Pereira
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-07-21 |
| Examining board members: |
Luis Carlos de Souza Ferreira;
Rafael Ciro Marques Cavalcante;
Edison Luiz Durigon;
Karina Inácio Ladislau de Carvalho Salmazi
|
| Advisor: | Luis Carlos de Souza Ferreira |
| Abstract | |
Dengue is a disease caused by dengue virus (DENV) whose incidence is alarming in the world. Its main method of control, combating the vector, is not fully effective, which makes the search for safe and effective vaccines a priority. Moreover the use of non-structural proteins of the virus shows promise for the development of vaccines against dengue. In this work we developed and characterized vaccine strategies based on the NS1 protein using the intradermal route (i.d.) immunization. For this, chimeras containing the NS1 protein fused to DENV2 antibodies specific for the receptor DEC and DCIR2 dendritic cells were obtained. The use of these proteins in immunization trials by way i.d. and i.p. induced NS1-specific humoral responses, with modulation of the IgG subclasses profile, increased affinity thereof without causing adverse effects in the immunized animals. Thus, the new combination of targeting strategy described here with via i.d. any be promising for the development of vaccines against DENV. (AU) | |
| FAPESP's process: | 13/26942-2 - Development of a vaccine formulation against dengue based on the nonstructural protein 1 (NS1) administered by the intradermal route |
| Grantee: | Lennon Ramos Pereira |
| Support Opportunities: | Scholarships in Brazil - Master |