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| Author(s): |
Ezequiane Machado da Silva
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2016-08-26 |
| Examining board members: |
Hosana Maria Debonsi;
Angela Regina Araujo;
Leonardo Gobbo Neto;
Adriana Aparecida Lopes;
Dioneia Camilo Rodrigues de Oliveira
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| Advisor: | Hosana Maria Debonsi |
| Abstract | |
Marine organisms are recognized as a rich reservoir of natural products with exceptional molecular structures. In this context, cyanobacteria and endophytic fungi has emerged as source of promising bioactive compounds. The aim of this work was to look for biologically active compounds from the marine cyanobacteria strains, collected in Ubatuba, coast of the São Paulo state (Brazil), and to explore the biosynthetic potential of the fungus Trichoderma atroviride (endophyte isolated from marine seaweed Bostrychia tenella, collected in the rocky Shore of Praia de Fortaleza, Ubatuba, SP) by coculture with the cyanobacteria strain Geitlerinema sp CENA556. Initially, five species of marine cyanobacteria were isolated from the sample collected at the coast of São Paulo, and then characterized phylogenetically. Among them, the strains coded as Geitlerinema sp CENA552 and Geitlerinema sp CENA556 were cultured in mimicking seawater medium and extracted with a mix of CH2Cl2/ MeOH (2:1), and then EtOAc. The concentrated extract was partitioned into hexane fraction and MeOH/H2O (95: 5) fraction. Four known compounds were identified from the hexane fraction of the Geitlerinema sp CENA552 extract by GC-MS analysis: 2-hexyl-1-decanol, 3-octadecene, neophytadiene, and methyl palmitate. Similarly, the compounds dodecanal, 8-heptadecene, palmitaldehyde, neophytadiene, phytol, methyl palmitoleate, methyl 7-hexadecenoate, and methyl 9-hexadecenoate were identified from the hexane fraction of the Geitlerinema sp CENA556 extract. Starting of MeOH/H2O (95: 5) fraction of Geitlerinema sp CENA556 extract, seven compounds were isolated by semi preparative HPLC-PDA and identified by NMR spectroscopy and HR-MS analysis. Among them, five known nucleosides first isolated from cyanobacteria: 2\'-deoxyuridine, thymidine, adenosine, 2\'-deoxyadenosine, and uridine. Also two amino acids: D-leucine and L-phenylalanine. Trichoderma atroviride was grown alone in rice and Czapeck media and the coculture between the fungus and the cyanobacteria (Geitlerinema sp CENA556) were performed using the same media. The CH2Cl2/ MeOH (2:1) and EtOAc pooled extract from the coculture of the fungus T. atroviride and the cyanobacteria Gleiterinema sp CENA556 in Czapek medium shows significant difference in the metabolites production when compared to individually cultured fungus and cyanobacteria. Four catechol type siderophores were isolated from MeOH/H2O (95: 5) fraction of this extract: agrobactin, agrobactin A, photobactin and one novel compound. Agrobactin, the major compound, showed substantial antibacterial activity against P. mirabilis, E. coli, S. saprophyticus, S. aureus and P. aeruginosa. Thus, the studied brazilian cyanobacteria Geitlerinema sp appeared to be promising for isolation of nucleosides with biological activity. Furthermore, the fungus T. atroviride proved to be a prolific source of antibacterial siderophores obtained by biological elicitation with the cyanobacteria Geitlerinema sp CENA556. (AU) | |
| FAPESP's process: | 12/13841-0 - Chemical and pharmacological exploration in marine cyanobacteria |
| Grantee: | Ezequiane Machado da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |