Full text
| |
| Author(s): |
Paulo César Ferreira dos Santos
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-11-03 |
| Examining board members: |
Jose Maria Alvarez Mosig;
Maristela Martins de Camargo;
Cláudio Vieira da Silva;
Tatiana Takiishi
|
| Advisor: | Jose Maria Alvarez Mosig |
| Abstract | |
Chronic Chagasic Cardiomyopathy (CCC) is the most morbid element of Chagas Disease, the elucidation of its physiopathology being fundamental. However, little is known about the role of structural cells, such as cardiomyocytes, in the course of the disease. In other cardiac pathologies, it has been shown that IFN-γ determines the priming of several resident populations, positively modulating their response. In this work, HL-1 cardiomyocytes and C3H/HePas mice were primed with IFN-γ (in brief or extended protocols) and challenged with LPS, the cytokines produced being measured in the supernatants. We observed that IFN-γ positively modulates the in vitro production of many cytokines by HL-1 cells (IP-10, MCP-1, G- CSF, RANTES, MIG, IL-6, MIF) and also their in vivo production at the heart (IP-10, KC, G-CSF, LIF and IL-6). Besides, IFN-γ was able to decrease the LPS-induced production of VEGF and GM-CSF by HL-1 cells. Our data allow us to conclude that cardiomyocytes actively participate in the inflammatory response of the heart, being sensitive to products released by professional immune cells. This work may serve as a basis for further studies on the profile of the cytokines secreted in the heart tissue along the course of cardiac inflammation. (AU) | |
| FAPESP's process: | 14/17586-0 - MODULATION OF CARDIOMYOCYTE SIGNALING BY PRIMING WITH CITOKINES |
| Grantee: | Paulo César Ferreira dos Santos |
| Support Opportunities: | Scholarships in Brazil - Master |