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| Author(s): |
Janaína Capelli Peixoto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-11-28 |
| Examining board members: |
Sirlei Daffre;
João Trindade Marques;
Sergio Daishi Sasaki;
Aparecida Sadae Tanaka;
Mauro Javier Cortez Véliz
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| Advisor: | Sirlei Daffre |
| Abstract | |
The cattle tick Rhipicephalus microplus is the vector of the bacteria Anaplasma marginale, the etiological agent of bovine anaplasmosis. This study aimed to evaluate the molecular and functional role of the signaling pathways Toll, Imd, Jnk, and Jak/Stat in R. microplus. An in silico analysis allowed the identification of several genes of these signaling pathways. In addition, there was a negative modulation of expression of genes of these pathways in the tick cell line BME26 upon infection with A. marginale. The silencing of some transcription factor genes via RNA interference in ticks indicated that the transcription factor Relish (from the Imd pathway) was involved in the control of bacterial infection, possibly via regulation of the expression of the antimicrobial peptide microplusin. This study helped elucidate the role of the immune system of ticks in the vector-pathogen interface. (AU) | |
| FAPESP's process: | 11/23549-2 - Signaling pathways involved in the immune response of the cattle tick Rhipicephalus microplus |
| Grantee: | Janaína Capelli Peixoto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |