Effect of albumin modified by advanced glycation in gene expression of SLC2A4 in skeletal muscle.

Author(s)

Danilo Antônio Corrêa Pinto Júnior

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-12-01

Examining board members

Ubiratan Fabres Machado; Fernanda Ortis; Marisa Passarelli; Marilia Cerqueira Leite Seelaender; Leonardo dos Reis Silveira

• Advisor: Ubiratan Fabres Machado

Abstract

The participation of advanced glycation end products (AGEs) in the diabetes-related chronic complications has been extensively investigated. However, little is known about AGEs participation in glycemic homeostasis, for which the glucose transporter GLUT4 (Slc2a4 gene) plays a key role. The aim of this study was indentify the effect of AGEs in an in vivo and in vitro models in Slc2a4/GLUT4 expression. In vivo and in vitro models showed decrease of Slc2a4/GLUT4 expression and insulin sensitivity (only on in vivo model). AGEs increase inflammatory and endoplasmic reticulum stress ways by NFKB and chaperones respectively. In sume, The results reveal that AGEs repress Slc2a4/GLUT4 expression in muscle, in a reticulum endoplasmic stress- and inflammatory-mediated way. This effect contributes to impair plasma glucose clearance, highlighting AGEs reduction/inhibition as a target to improve glycemic control in diabetes. (AU)

• FAPESP's process: 12/15514-7 - Effect of advanced glycated albumin upon Slc2a4 gene in skeletal muscle
• Grantee: Danilo Antônio Corrêa Pinto Júnior
• Support Opportunities: Scholarships in Brazil - Doctorate