Characterization of the mechanisms of CspC action in Caulobacter crescentus cell ...
Study of the role of CspD in Caulobacter crescentus stationary phase
Study of the role of the DEAD-box RNA helicase RhlB in the RNA stability and gene...
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Author(s): |
Juliana da Silva Santos
Total Authors: 1
|
Document type: | Doctoral Thesis |
Press: | São Paulo. |
Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
Defense date: | 2016-04-05 |
Examining board members: |
Marilis do Valle Marques;
Regina Lúcia Baldini;
Rodrigo da Silva Galhardo;
Frederico José Gueiros Filho;
Marcia Giambiagi de Marval
|
Advisor: | Marilis do Valle Marques |
Abstract | |
Point mutations in the CspC CSDs caused a more severe phenotype than that of the null strain. None of the C. crescentus CSPs complemented the cold sensitivity of E. coli BX04 mutant. However, the individual CSDs of C. crescentus CspC complemented this phenotype. A microarray global transcriptional profiling showed the absence of cspC affected the transcription of 11 genes at exponential phase and 60 genes at stationary phase. mRNA decay experiments showed that the sciP, aceA e CC0682 mRNAs were less stable in the cspC mutant, indicating that its effect could be at least partially due to posttranscriptional regulation. (AU) |