Low fluoride dentifrice and deciduous enamel demineralization under different cariogenic challeng and sucrose exposure

Author(s)

Regiane Cristina do Amaral

• Document type: Master's Dissertation
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2010-02-25

Examining board members

Jaime Aparecido Cury; Cecilia Claudia Costa Ribeiro; Maria da Luz Rosário de Sousa

• Advisor: Jaime Aparecido Cury

Abstract

The use of fluoride dentifrice with low concentration (500 ppm F) has been recommended for children due to the fluorosis risk, but it effectiveness in anti-caries compared to conventional dentifrice with fluoride concentration (1100 ppm F) is unknown when accumulation of dental biofilm and different sucrose exposure. Thus, an in situ study, crossed, double-blind in relation to the dentifrices and split-mouth considering the frequency of sucrose exposure was conducted in 4 phases of 14 days each. In each phase, 14 volunteers used intra-oral palatal appliances containing 8 blocks of deciduous enamel with pre-determined surface hardness, which were submitted to the accumulation of dental biofilm and exposed to 20% sucrose in the frequencies of the 2 to 8x/day. Three times a Day the volunteers used dentifrices formulations with 500 or 1,100 ppm F (NaF, silica-based). On the 14th day, the biofilm formed on the enamel blocks was collected before and 15 min after brushing with the respective dentifrice for determination of fluoride concentration in the biofilm fluid and solids. Enamel demineralization was assessed by surface hardness loss (%SHL) and cross-sectional hardness (_S = area of hardness loss). F uptake by enamel was also determined. F concentration in fluid and solid part of the biofilm was statistically higher when 1,100 ppm F dentifrice was used (p<0.05). The dentifrices did not differ statistically in terms of %SHL, _S and F in enamel (p>0.05), but only the conventional F dentifrice significantly reduced caries lesion progression according to the frequency of sucrose exposure (p<0.05). The findings suggest that the high F availability in biofilm, resulting from the use of conventional compared to low F dentifrice, is important to reduce caries lesion progression under a high frequency of sucrose exposure. (AU)

• FAPESP's process: 07/05994-3 - Low fluoride dentifrice and deciduous enamel demineralization under high cariogenic challenge
• Grantee: Regiane Cristina Do Amaral
• Support Opportunities: Scholarships in Brazil - Master