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Development of immature of species of forensic importance, Chrysomya megacephala (F.) and Chrysomya putoria (W.) (Diptera: Calliphoridae), under influence of different temperatures and / or fluoxetine hydrochloride

Full text
Author(s):
Marcela Aquiyama Alonso
Total Authors: 1
Document type: Master's Dissertation
Press: Campinas, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
Defense date:
Examining board members:
Patricia Jacqueline Thyssen; Arício Xavier Linhares; Claudio José Von Zuben
Advisor: Patricia Jacqueline Thyssen
Abstract

Insects are the most diverse and abundant group of the Animal Kingdom, with great diversity of morphological, physiological and feeding habits and are found in nearly all habitats and ecosystems. Scavengers species can provide important information of forensic interest, as the post-mortem interval (PMI) estimate, based on, e.g., the age of the larvae reared in decomposing bodies. The development of these insects can be affected by temperature oscillation and presence of toxic substances, among other factors, on the rearing media. Chrysomya megacephala (F.) and Chrysomya putoria (W.) (Diptera: Calliphoridae), introduced in Brazil, are considered of forensic, medical and veterinary importance, due to their necrophagous and synanthropic behaviour. This study evaluated the developmental time of C. megacephala and C. putoria under different temperatures and / or with fluoxetine hydrochloride, an antidepressant drug, in rabbit liver, the rearing substrate. The relationship between temperature and development, on the embryonic phase, was similar for both species. Egg developmental time for C. megacephala was approximately of 64-7h at 13 and 35 °C, respectively, and for C. putoria was 69-8h at 13 and 35 °C, respectively. The larval hatching occurred at 13 °C, but, at this temperature, the larval development was not completed. Both temperature and fluoxetine hydrochloride, when present, affected the development of the larvae. For both species, the larvae of control group completed their development 24h faster than the fluoxetine hydrochloride group at 17 °C, but the development was 12h slower at 35 °C. Studies considering real egg developmental time and evaluating, simultaneously, the insects' response for two or more variables that might influence their development are of great value to increase the accuracy of PMI estimate (AU)

FAPESP's process: 13/07022-0 - Development of immature species of forensic importance, Chrysomya megacephala (F.) and Chrysomya putoria (W.) (Diptera: Calliphoridae), under the influence of different temperatures and / or fluoxetine hydrochloride
Grantee:Marcela Aquiyama Alonso
Support Opportunities: Scholarships in Brazil - Master