Distribution of EGF, EGFr, TGF-'beta'1, IL-1'alfa' and CSF-1 in the periodontum of rat lower incisors, in normal and altered functional conditions

Author(s)

Silvana Pasetto

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2005-02-25

Examining board members

Pedro Duarte Novaes; Darcy de Oliveira Tosello; Miralva Aparecida de Jesus Silva; Victor Elias Arana Chaves; Darcy de Oliveira Tosello; José Merzel

• Advisor: Pedro Duarte Novaes

Abstract

The aim of this study, was to analized, using immunohistochemical technique, the distribution of EGF, EGFr, TGF-b1, IL-1a e CSF-1, in periodontal tissues of three regions of rat lower incisors, in normal and altered functional conditions. Fifty animals, had their left lower incisors, cut at the level of the interdental papilla. Becoming out occlusion, they are considered hipofunctional teeth. The contralateral incisors, attrited with both upper incisors are considered hiperfunctional teeth. Twenty-five of these animals were killed 8 hours after. In the order twenty-five animals, the shortening was repeated every second day, and killed 8 days after. Ten animals were kept in normal functional conditions. The hemimandibules of all animals were fixed in Karnovsky (with 0,1% of glutaraldehyde), demineralized with EDTA, subdivided in five transversal regions and embedded in paraplast. Cross sections, 4 mm-thick were collected in glass slides coated with silane. The antibodies were developed with DAB plus H2O2. The dental follicle and the periodontal ligament showed variation in the staining intensities, for all antibodies used, among the different regions and groups analyzed. Apparently, however, the periodontal tissues of the incisors, after eight days of hipofunction, showed stronger immunoreactivity, particularly with CSF-1, as compared with incisors under normal and hiperfunctional conditions. The results suggest that this molecular ¿cascade¿, may be related with the enlargement of the rodent incisor eruptive pathway, since these teeth grow for a long period of animal¿s life (AU)