IL-6, TNF-'alfa' and MCP-1 production and expression by fibroblasts (3T3) and odontoblasts (MDPC-23) after oral bacteria exposure

Author(s)

Carolina Carvalho de Oliveira Santos

• Document type: Doctoral Thesis
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2015-05-28

Examining board members

Alexandre Augusto Záia; Ericka Tavares Pinheiro; Flaviana Bombarda de Andrade Ferreira; José Flávio Affonso de Almeida; Caio Cezar Randi Ferraz

• Advisor: Alexandre Augusto Záia

Abstract

Dental pulp contains a heterogeneity of cells in its composition, among them stand out from fibroblasts and odontoblasts. When this tissue is attacked by bacteria or their by-products, fibroblasts and odontoblasts can secrete several inflammatory cytokines in order to attract and activate immune system to contain infectious agents. TNF-'alfa', IL-6 and MCP-1 are proteins secreted by fibroblasts and odontoblasts mainly involved in the origin and activation of the inflammatory process. This study aimed to evaluate gene expression and secretion of inflammatory cytokines after in vitro contact of bacteria Sreptococcus mutans, Porphyromonas gingivalis, Enterococcus. faecalis and its by-products with odontoblasts and fibroblasts. The proteins were quantified by flow cytometry type CBA and gene expression by Real Time - PCR in periods of 4, 8 and 24 hours. The results showed that the studied bacteria promoted sensitizing cells for expression and production of the cytokines IL-6, TNF-'alfa', MCP-1, particularly during the first 8 hours of contact. Both direct contact with bacterias or their by-products resulting in stimulation to the cells, however, direct contact with the bacteria S. mutans and E. faecalis was more efficient for stimulating expression and cytokine production. On the other hand, the indirect contact with P. gingivalis shown to be more effective to stimulate the production of cytokines. Thus, we may conclude that fibroblasts and odontoblasts are able to express and produce proinflammatory cytokines from different contacts when stimulated by different bacterial types (AU)

• FAPESP's process: 11/08360-0 - Analysis of bacterial effect from endodontic infection and their byoproducts in inflammatory cytokine and chemokine expression by fibroblasts and odontoblasts-in vitro
• Grantee: Carolina Carvalho de Oliveira Santos
• Support Opportunities: Scholarships in Brazil - Doctorate