Phylogenetic analysis of authochtonous bovine respiratory syncytial virus isolates and improvement of an experimental model of infection in mice

Author(s)

Fernando Rosado Spilki

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2006-11-17

Examining board members

Clarice Weis Arns; Edel Figueiredo Barbosa Stancioli; Paulo Michel Roehe; Marcelo Brocchi; Maria Silvia Viccari Gatti

• Advisor: Clarice Weis Arns

Abstract

Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in young cattle. The virus is a member of the Paramyxoviridae family, Pneumovirinae subfamily, belonging to the Pneumovirus genus. During the last fifteen years, serological evidence and isolation of the virus revealed that BRSV is circulating in Brazil, causing clinically evident respiratory disease or subclinical fonns of the infection. In the first part of the present work, susceptibility of six different cell lines to BRSV'"li infection in regard to viral isolate variability and growth characteristics of the virus were examined. Chicken embryo related cells (CER), and bovine CRIB cells (a bovine viral diarrhea virus-resistant clone ofMDBK cells) showed to be the most appropriate for virus multiplication. Both cells provided infectious virus titres ofup to 105,5 TCID50 (50% tissue culture infective doses per 100 flL)...Note: The complete abstract is available with the full electronic digital thesis or dissertations (AU)