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| Author(s): |
Juliana Florenzano Martorelli
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-05-29 |
| Examining board members: |
Soraia Kátia Pereira Costa;
Maria Helena Catelli de Carvalho;
Sandra Helena Poliselli Farsky;
Wothan Tavares de Lima;
Walter dos Reis Pedreira Filho
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| Advisor: | Soraia Kátia Pereira Costa; Lucia Rossetti Lopes |
| Abstract | |
The mice exposure to 1,2-naphthoquinone (1,2-NQ), during postnatal period induced increased susceptibility of males (but not females) to asthma. The aim of this study was to investigate the intensity of lung damage to impact of 1,2-NQ on young mice of both sexes, and to investigate the associations between TRPA1 and 1,2-NQ. Male and female C57Bl/6 mice (2-5g) were exposed to 1,2-NQ (100 nM). After 24 h postnatal exposure to 1,2-NQ, only female lungs showed increased catalase activity and Nrf2 mRNA expression. The allergic stimuli at puberty led to increased glutathione peroxidase, reductase and S-transferase activities only in female lung, which, unlike male, did not exhibit exacerbation of asma, but showed increased pulmonary nitration and protein carbonylation, and increased mRNA expression of eNOS and TRPA1. The TRPA1 antagonist reduced eosinophilia in male lung and inhibited the increased [Ca2+]i in dorsal root ganglion neurons culture to 1,2-NQ. The lack of susceptibility in female might be linked to increased antioxidant defenses and the pulmonary maturity. (AU) | |
| FAPESP's process: | 13/02115-0 - Role of TRPA1 receptors in the distinct susceptibility to lung allergic inflammation in both mice gender exposed to pollutant in neonatal phase |
| Grantee: | Juliana Florenzano Martorelli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |