Study of the role of CspD in Caulobacter crescentus stationary phase.

Author(s)

Julia Albano de Barros

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2017-08-29

Examining board members

Marilis do Valle Marques; Andrea Balan Fernandes; Cristina Elisa Alvarez Martinez; Aline Maria da Silva

• Advisor: Marilis do Valle Marques

Abstract

In order to elucidate if CspD in Caulobacter crescentus has the same role as in E. coli, we analyzed the effect of overexpressing CspD in bacterial growth and concluded that opposite from what its observed in Escherichia coli, it didnt affect growth. Yet, when over expressed in E. coli, CspD did not show any effect on the bacterial growth. Through two-dimensional gel electrophoresis of the cspD null mutant, it was possible to investigate if the protein is affecting the expression pattern of the cell. Along with ClpP protease, enzymes that are involved in the purine and amino acid metabolism are among those that showed differential expression in the absence of cspD. The data obtained from the two-dimensional gel electrophoresis were validated through qRT-PCR. The binding of CspD to nucleic acids was investigated. However, no binding of the protein to the nucleic acids was observed. Finally through western blotting assays, it was possible to conclude that CspD is post- translationally regulated by the Lon protease. (AU)

• FAPESP's process: 15/10919-7 - Study of the role of CspD in Caulobacter crescentus stationary phase
• Grantee: Julia Albano de Barros
• Support Opportunities: Scholarships in Brazil - Master