Effect of experimental infection with bovine viral diarrhea virus in gestation and newborn piglets

Author(s)

Daniele Araujo Pereira

• Document type: Doctoral Thesis
• Press: Jaboticabal. 2018-05-11.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2018-04-10
• Advisor: Luís Guilherme de Oliveira

Abstract

BVDV belongs to the genus Pestivirus, and can cause reproductive problems, however, there is still a lack of research to clarify the pathogenicity in different gestational periods of gilts and the effects in the neonates. For the study, 12 gilts divided into groups (G) were experimentally inoculated with the strain BVDV-2 (VS-253) at the dose 106,85TCID50 by the oronasal route, one group with 30 days before insemination (G0; n=2) and three groups during gestation, first (G1;n=2), second (G2;n=3), last third (G3;n=3) and the fifth control group (G4;n=2). Samples of blood and nasal swabs from the gilts were collected every three days from the day of inoculation until the day of delivery for virusneutralization test, quantitative PCR (qRT-PCR), blood count. On the day of delivery, 40% of the neonates were euthanized to obtain tissue samples at necropsy for histopathology and qRT-PCR. The sows were seroconverted between 12 and 33 days after inoculation and the virus was detected in the blood between 3 and 12 days, and in the nasal swab between 6 and 24 days in the G0, G1, G2 and G3 sows after inoculation, but it was not possible to detect BVDV in piglet tissues and no significant alterations were found through histopathology. The mean and standard deviation of the mean cycles (Cq) of the blood samples (Cq = 34.87 ± 0.60) and nasal swab (Cq = 34.61 ± 0.87) of the sows corresponded between approximately 107 to 490 TCID50/mL, obtaining a low viral load in swines, and transplacental infection was`t possible. (AU)

• FAPESP's process: 15/08531-0 - EFFECT OF DIARRHEA VIRUS INFECTION IN BOVINE VIRAL PREGNANCY SWINE AND NEONATES
• Grantee: Daniele Araujo Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate