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| Author(s): |
Lívia Maísa Guiraldi
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2016-07-29. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu |
| Defense date: | 2016-07-22 |
| Advisor: | Simone Baldini Lucheis; Virgínia Bodelão Richini Pereira |
| Abstract | |
The family Trypanosomatidae includes etiological agents responsible for causing diseases in humans and other animals. There are dozens of protozoa belonging to different genres, especially protozoa representatives of Leishmania genre, which causes leishmaniasis and Trypanosoma, responsible for trypanosomiasis. While the transmission of leishmaniasis is due to the bites of female sand flies belonging to the genus Phlebotomus in the Old World and Lutzomyia in the New World, the transmission of trypanosomiasis depends on the species of trypanosome involved in the infection. It may occurs by the bite of adult insects of the genus Glossina or by the feces of insects, especially by the genre Triatoma and mechanically by bloodsucking flies, especially the Stomoxys genre. In addition to humans, domestic and wild animals can act as trypanosomatids reservoirs, including non-human primates, which are associated with the enzootic cycle of leishmaniasis and trypanosomiasis; the natural infection by Trypanosoma and Leishmania protozoa in wild mammals is common in nature. The aim of the study was to investigate trypanosomatids in captive primates coming from Municipal Zoological Park of Bauru by antibodies against the parasite and the amplification of the DNA of the parasite. For this, blood samples were collected from 39 primates. The molecular technique of Polymerase Chain Reaction (PCR) was applied in research for Leishmania braziliensis, Leishmania amazonensis and Leishmania infantum using species-specific primers directed to the region of kDNA parasites; all animals were negative by this technique. However, with the use of primers for the ribosomal region (ITS-1) specific to Trypanosomatidae family, 37 of 39 (94.9%) animals were positive for Trypanosoma spp. It was also conducted serological evidence by the Immunofluorescente Antibody Test (IFAT) for Leishmania braziliensis, Leishmania infantum e Leishmania amazonensis, revealing one (01) animal Erythrocebus patas (patas monkey) positive for Leishmania braziliensis, with titer 160. The results show that primates from Municipal Zoological Park of Bauru are susceptible to infection by trypanosomatids, alerting the need for active search for sandfly vectors and triatomines in the grounds and surrounding areas, as a preventive measure for the risk of infection for staff and visitors. (AU) | |
| FAPESP's process: | 14/12187-0 - Cutaneous and visceral leishmaniasis in non-human primates from Bauru Zoo, SP |
| Grantee: | Lívia Maísa Guiraldi |
| Support Opportunities: | Scholarships in Brazil - Master |