Acoustic radiation force impulse elastography doppler and contrast-enhacd ultrassonography of dogs spleen

Author(s)

Marjury Cristina Maronezi

• Document type: Master's Dissertation
• Press: Jaboticabal. 2016-03-04.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2016-02-12
• Advisor: Marcus Antônio Rossi Feliciano; Wilter Ricardo Russiano Vicente; Júlio Carlos Canola

Abstract

The spleen is an organ develops much important functions in the body and the spleen is one of the main organs prone to the development of primary tumors, metastasis and a broad range of diseases of the hematopoietic system perform assessment of spleen is important. In veterinary medicine, principally in dogs because of economic importance, affective and scientific similarity to humans. However, the most of the methods used for conclusive diagnosis of splenic diseases are invasive methods, noninvasive lose in sensitivity and specificity difficulting proceed to a correct diagnosis. Thus, this study aimed to perform the ultrasound scan of dogs spleen using the elastographic (qualitative and quantitative) Doppler and contrast enhanced ultrasonography. Determining values not described in veterinary, which provide sufficient subsidies to get a conclusive diagnosis of the splenic condition in this species. The results in healthy dogs showed, all splenic segments visualized with the B-mode ultrasonography appeared normal. Different splenic segments examined with qualitative elastography were free of any detectable malformations and the images appeared as homogeneous dark areas. The mean shear velocity values were 2.32 m/s for head, 2.16 m/s for body and 2.25 m/s for tail of the spleen, and they did not vary between the different age groups (P>0.05). In dogs with ehrlichiosis in subclinical phase showed, by B-mode examination, observed splenomegaly with presence of rounded borders and heterogeneous echotexture and mixed echogenicity in spleen. For Doppler found values for vascular indices of the splenic artery: systolic velocity: 22.59 ± 8.07 cm/s; diastolic velocity: 5.25 ± 4.66 cm/s; and resistance index: 0.71 ± 0.14. For contrast enhanced ultrasonography observed values for wash-in: 5.31 ± 0.7s, peak enhancement: 18.56 ± 2.90s and wash-out: 94.56 ± 35.21s. The ARFI elastography is a new method and it may aid in the diagnosis and evaluation of splenic abnormalities routinely assessed in veterinary practice. The conventional ultrasonography of the spleen in dogs with ehrlichiosis associated with hemodynamic evaluation by Doppler and contrast enhanced ultrasonography are important tools for diagnosis trial and could help the monitoring clinical evolution in subclinical phase. (AU)

• FAPESP's process: 13/26197-5 - Study of the splenic stiffness by Acoustic Radiation Force Impulse (ARFI) elastography in healthy canines
• Grantee: Marjury Cristina Maronezi
• Support Opportunities: Scholarships in Brazil - Master