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| Author(s): |
Carlos Henrique Silveira Rabelo
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2016-09-06. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2016-08-26 |
| Advisor: | Ricardo Andrade Reis |
| Abstract | |
Our objective was to determine the impact of Lactobacillus and Bacillus subtilis as silage additives on the quality of corn silage and their effects on the performance of beef cattle and sheep. For that, three studies were carried out. The first study investigated the effect of L. buchneri as a silage inoculant or probiotic on in vitro ruminal fermentation of corn silage. The experiment was carried out under a 2 × 3 factorial arrangement, with two silages (untreated or treated with L. buchneri) as substrate combined with three ruminal fluids obtained from the wethers consuming the three different diets described in the second study. The results appointed that L. buchneri as a silage inoculant alters fermentation patterns of corn silage leading to an increase in gas production over time; however, this increase is not accompanied by increased organic matter digestibility or total volatile fatty acids concentration. Conversely, the utilization of L. buchneri as a probiotic appears to have a greater impact on fermentation end products than in vitro gas production, particularly during earlier stages of fermentation (i.e., up to 9 h of fermentation). The second study investigated the effect of L. buchneri as a silage inoculant or probiotic in wethers. Six cannulated-wethers were arranged in a double 3 × 3 Latin square of three 19-d periods, and they were fed a total mixed ration ad libitum using a 70:30 forage:concentrate ratio. Diets were composed of 1) untreated corn silage; 2) inoculated corn silage; and 3) untreated corn silage with a daily dose of L. buchneri applied directly into the rumen of the wethers. L. buchneri as a silage inoculant led to changes in fermentation and chemical composition of corn silage, and increased dry matter intake with additional minor shifts in the relative proportion of Ruminococcus flavefaciens and ruminal fermentation of wethers. Conversely, L. buchneri as a probiotic led only to a minimal shift in the relative proportion of R. flavefaciens. The third study investigated the effect of combining Lactobacillus plantarum either with Lactobacillus buchneri (LBLP silage) or B. subtilis (BSLP silage) for corn silage on the growth performance of finishing feedlot beef cattle. Thirty six Nellore × Brown Swiss crossbred bulls (initial body weight of 316 ± 33.9 kg) were fed a total mixed ration ad libitum using a 40:60 forage:concentrate ratio for 89 d post-adaptation. Bulls (n = 12) were distributed in a completely randomized design for one of three diets containing untreated, LBLP and BSLP corn silage. Silage inoculation unaffected feed intake and growth performance of bulls, but depressed apparent digestibility of the diets. Overall, the results of the three experiments revealed that bacterial inoculants were not able to improve the fermentation process of corn silage and the performance of animals fed corn silage-based diet. (AU) | |
| FAPESP's process: | 12/25463-0 - Action of lactic-acid bacterial and Bacillus subtilis in manipulation of fermentative process in corn silage and their effect on performance animal |
| Grantee: | Carlos Henrique Silveira Rabelo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |