Bone marrow aspirate and platelet-rich plasma combined or not with low-level laser therapy on bone healing: a histomorphometric and immunohistochemical study

Author(s)

Eduarda de Lima Graciano Belem

• Document type: Master's Dissertation
• Press: Araçatuba. 2016-09-22.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2016-08-26
• Advisor: Maria José Hitomi Nagata

Abstract

This study evaluated the influence of bone marrow aspirate (BMA) and platelet-rich plasma (PRP) combined or not with low-level laser therapy (LLLT) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. 96 rats were divided into 6 groups: C, PRP, BMA, LLLT, PRP/LLLT and BMA/LLLT. A 5 mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In groups PRP and BMA, the defects were filled with PRP and BMA, respectively. In Group LLLT, the defect received laser irradiation (InGaAlP laser), was filled with blood clot and irradiated again. In groups PRP/LLLT and BMA/LLLT, the defect received laser irradiation (InGaAlP laser), was filled with PRP or BMA, respectively, and irradiated again. Animals were euthanized at either 10 or 30 days postoperative. Histomorphometric and immunohistochemical analyses were performed. Newly formed bone area (NFBA) was calculated as a percentage of the total area of the original defect. Proliferating cell nuclear antigen (PCNA), bone morphogenetic protein 2/4 (BMP-2/4) and osteocalcin (OCN) immunohistochemical staining were performed. PCNA-positive, BMP-2/4-positive and OCNpositive cells were quantified. Data were statistically analyzed. At 10 days postoperative, Group BMA presented significantly greater NFBA than control. At 30 days postoperative, Group BMA presented significantly greater NFBA than control and Group LLLT, as well as significantly higher numbers of BMP-2/4-positive and OCN-positive cells than control. Within the limits of this study, it can be concluded that the treatment with BMA promoted bone regeneration in critical-size rat calvarial defects at both 10 and 30 days postoperative. (AU)

• FAPESP's process: 14/02094-5 - Bone marrow aspirate and platelet-rich plasma combined or not with low-level laser therapy on bone healing: a histomorphometric and immunohistochemical study
• Grantee: Eduarda de Lima Graciano Belem
• Support Opportunities: Scholarships in Brazil - Master