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| Author(s): |
Miriane Carneiro Machado Salgado
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São José dos Campos. 2017-01-11. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos |
| Defense date: | 2016-11-09 |
| Advisor: | Yasmin Rodarte Carvalho |
| Abstract | |
Excessive alcohol consumption is an important public health problem and also has direct relation with bone loss by imbalancing bone turnover. Estrogen deficiency is directly associated with osteoporosis, because it leads to an unequal bone resorption and formation by increased osteoclast formation and decreased synthesis of osteoblasts. The combination of osteoporosis and consumption of alcohol can have deleterious and synergistic effect on bone tissue and has been the subject of several studies. The objective of this study will be to investigate possible changes in bone metabolism in femurs of rats submitted to chronic alcoholism and estrogen deficiency induced by ovariectomy. We used 90 rats (Rattus norvegicus, Albinus variation, Wistar) with 3 months old. The animals were divided into six different groups of equal number, according to the type of diet and the presence or absence of hormones: Group 1: sham ovariectomized (sham), water and free diet, Group 2: Sham treated daily with alcoholic solution of 20% and feed ad libitum; Group 3: Sham isocaloric nutritional control group – treated with liquid diet containing sucrose with the same average calories ingested on the eve by the alcohol group and solid diet; Group 4: ovariectomy, water and solid diet free; Group 5: ovariectomy treated daily with alcoholic solution of 20% and feed ad libitum; Group 6: ovariectomy isocaloric nutritional control group like group 3. After 8 weeks from the beginning of the diet, all animals were sacrificed and femurs were removed. Diet analysis showed that the Ovx ad libitum was the group that ingested more feed and gained more weight, showing significant differences with the other groups. The animals of alcohol groups, consuming on average 16 grams of alcohol per day, Sham alcohol group consumed more alcohol, as compared to OVZ alcohol. Femurs were evaluated by histomorphometric analysis and immunohistochemical analysis, using the markers, RANKL, Osteoprotegerin and Osteocalcin. The histomorphometric analysis evidenced that ovariectomized animals showed, in percentage, a lower amount of trabecular, than the sham operated, but without significant differences. Markers of bone turnover in immunohistochemical reactions showed no significant changes in bone remodeling process. It was concluded that the combination of estrogen deficiency and moderate chronic alcohol consumption did not cause deleterious effects on bone remodeling of the femur of young adult rats. (AU) | |
| FAPESP's process: | 13/19517-3 - Immunohistochemical analysis of femoral bone metabolism of rats submitted to alcohol consumption and/or estrogen deficiency |
| Grantee: | Miriane Carneiro Machado Salgado |
| Support Opportunities: | Scholarships in Brazil - Doctorate |