Optimization of Capsicum baccatum var. pendulum callus culture conditions

Author(s)

Tiago Fidemann

• Document type: Master's Dissertation
• Press: Assis. 2017-05-03.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências e Letras. Assis
• Defense date: 2017-03-09
• Advisor: Eutimio Gustavo Fernández Núñez; Mônica Rosa Bertão

Abstract

The oxidative stress and inflammations are associated with many diseases such as diabetes, cancer, cardiovascular and neurodegenerative diseases including atherosclerosis causing concern worldwide. As an alternative, the plants can play a key role due to their pharmacological properties against these disorders; these attributes are most of the times associated with the presence of polyphenolic compounds. In this context, peppers can be an alternative to obtain these compounds. However, the presence of secondary metabolites is low and affected by several abiotic factors. As an alternative to circumvent this problem, methodologies related to in vitro cultures of plant cells and tissues were applied. The objective of the present work was to standardize the extraction of explants (Chapter 1) to be used in a callus culture optimized through a systemic approach (Chapter 2). In Chapter 1, through a two-level full factorial experimental design (24 ) was numerically determined the effect of four factors [The solution type for osmotic conditioning (water and 1% KNO3 aqueous solution), germination medium (agar and agar + gibberellic acid at 1.88 mg.L -1 , GA3), post-sowing time (15 and 30 days) and two genotypes (Pitanga and Cambuci)] in cotyledon emergence and germination rates. The genotypes responses to the osmotic conditioning solution and to the germination medium were different. The optimal combination of treatments for in vitro germination and development of seedlings for Pitanga and Cambuci was water + agar and water + agar-GA3, respectively. In Chapter 2, aiming to optimize systemically the Cambuci pepper callus culture stage for higher biomass and secondary metabolites productionwere evaluated explant’s origin, plant growth regulator type and their concentrations through a multilevel factorial design. The values obtained in the optimized conditions were: callus mass (225.03 mg), antioxidant activity (35.95%), total phenols (11.48mg of GAE/g DE) and flavonoids (15.92 mg of RU/g DE). (AU)

• FAPESP's process: 14/26997-4 - Optimization of callus and suspension culture conditions for Capsicum baccatum var. pendulum cells
• Grantee: Tiago Fidemann
• Support Opportunities: Scholarships in Brazil - Master