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| Author(s): |
Jackeline Pinheiro Barros
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-04-03 |
| Examining board members: |
Beny Spira;
Rodrigo da Silva Galhardo;
Maria Inês Zanoli Sato;
Aline Maria da Silva
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| Advisor: | Beny Spira |
| Abstract | |
To adapt to various environmental conditions Escherichia coli regulates its gene transcription. An important regulator of bacterial adaptation is the sigma subunit of RNA polymerase, responsible for the transcription of most genes related to stationary phase and stress situations. The rpoS gene has a high degree of polymorphism, acquiring null or attenuation mutations in strains grown in the laboratory. In nature, however, nonfunctional rpoS alleles are apparently less common. In this work a temporal analysis of the profile of RpoS in environmental isolates of E. coli was carried out, in order to correlate the status of RpoS with the physico-chemical changes occurring throughout the year in wastewater. It was concluded that RpoS is highly persistent in the environment, and protein levels varied widely from one strain to another. There was no great correlation between the phenotypes and RpoS, but the data demonstrated the phenotypic diversity that may exist in the environment and that allow its survival for long periods outside the host. (AU) | |
| FAPESP's process: | 14/15330-9 - Analysis of the rpoS status in natural isolates of Escherichia coli |
| Grantee: | Jackeline Pinheiro Barros |
| Support Opportunities: | Scholarships in Brazil - Master |