Therapeutic L-asparaginase for acute lymphoid leukemia, purification employing alternative bioprocess

Author(s)

Louise Lacalendola Tundisi

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2017-07-28

Examining board members

Priscila Gava Mazzola; Ângela Faustino Jozala; André Moreni Lopes

• Advisor: Priscila Gava Mazzola

Abstract

L-asparaginase (L-ASNase) is a biopharmaceutical used mainly for acute lymphoblastic leukemia (ALL) treatment, which affects mostly children 3 to 5 years old and adolescents, increasing their survival from 20% to 90%. Obtaining a biopharmaceutical involves a series of processes ranging from its production to its purification. Purification of a biopharmaceutical, such as L-ASNase, generally consists of several steps which account for up to 80% of the total production costs, and is necessary to decrease its side effects when applied. As an extremely important step, the main objective of this work was to extract and purify the L-ASNase by alternative processes, using ethanol precipitation and two-phase aqueous systems. For this, the characterization of Aspergillus oryzae L-ASNase and the evaluation of the factors that affect its recovery and purification in a two-phase aqueous system were performed. Both stages are described in Chapters II and III of this work. The results obtained show that alternative processes can lead to a less steps and cheaper production of an extremely important drug in the treatment of ALL. With changes in the property of the systems, it is possible to modulate a partition of a molecule and being able to reach a high purification degree (AU)

• FAPESP's process: 16/01869-9 - Therapeutic l-asp for LLA purification employing alternative bioprocess
• Grantee: Louise Lacalendola Tundisi
• Support Opportunities: Scholarships in Brazil - Master