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Incorporation of Apigenin and tt-Farnesol into restorative materials

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Author(s):
Carolina Bosso André
Total Authors: 1
Document type: Doctoral Thesis
Press: Piracicaba, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
Defense date:
Examining board members:
Marcelo Giannini; Marcelo Henrique Napimoga; Fábio Dupart Nascimento; Vanessa Cavalli; Patricia Makishi
Advisor: Marcelo Giannini
Abstract

The aim of this in vitro study was to evaluate the incorporation of two anti-caries agents, Apigenin (Api) and tt-Farnesol (Far) into dental adhesives, a resin composite (CO), and a resin cement (CE), and its effect on the virulence of Streptococcus mutans, bonding performance to dentin, and mechanical properties of the CO and CE. Api (1 mM) and Far (5 mM) were incorporated separately, or in combination, into a self-etch adhesive (Clearfil S3 Bond Plus: CS3), and an each-and-rinse adhesive (OptiBond S: OPT). Api (5 mM) and Far (5 mM) were also incorporated separately or in combination, with or without fluoride (F), into CO and CE and compared to controls without additives. S. mutans was grown to form a biofilm on the surface of adhesive-coated hydroxyapatite, on CO or CE disks. Following 115 h of incubation, bacterial viability, dry-weight, polysaccharide composition (alkali soluble, water soluble and intracellular), and total protein content were quantified. Qualitatively SEM images from the biofilms were obtained. Bond strength was measured and SEM images of the dentin-adhesive interface were obtained. S. mutans biofilm was grown on the surface of class-I cavities restored using CO or CE. After incubation, was observed the carious demineralization and bacteria infiltration through a contraction-formed gap along the restoration margin, using confocal microscopy. In addition, the flexural strength and elastic modulus of CO and CE were determined. The adhesives, CS3 with Api or Api+Far, and OPT with Api or Far, reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide reduced with the addition of Api to CS3 and Far to OPT. Intracellular polysaccharide was also decreased by the addition of Api and Api+Far to CS3. No differences were observed in the total amount of protein or soluble polysaccharide. In addition, neither the bond strength nor the dentin-adhesive interfacial morphology was altered compared to the control groups. In the case of CO and CE, the bacterial viability reduced for CE with Api+Far+F. Dry-weight and insoluble and intracellular polysaccharides were reduced by the addition of Api, Api+Far or Api+Far+F for both materials. The amount of protein declined with the addition of Api or Api+Far to CO, and Api or Far to CE. No differences were observed in the soluble polysaccharides, flexural strength, elastic modulus, or bacteria infiltration for CO or CE. However, lower fluorescence of the remaining enamel was observed for both CO and CE groups with additives, and SEM images confirmed that the densest biofilms were observed in the control groups for all materials tested. CS3 with Api or Api+Far and, CO or CE with Api, Api+Far or Api+Far+F showed the greatest reduction of S. mutans virulence. The effect of incorporation of these natural compounds is material-dependent; however, the additives did not interfere with adhesive-bonding performance or with the mechanical properties of the CO or CE. No additive was able to completely suppress S. mutans activity, but the incorporation of Api and Far into restorative materials appears to be a promising approach for enhancing cariostatic properties when remaining enamel is present (AU)

FAPESP's process: 13/22823-9 - The influence of natural products incorporation on mechanical and antimicrobial properties of adhesive systems
Grantee:Carolina Bosso André
Support Opportunities: Scholarships in Brazil - Doctorate