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| Author(s): |
Karina Yanagui
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2016-08-29 |
| Examining board members: |
Gonçalo Amarante Guimarães Pereira;
Luiz Filipe Protasio Pereira;
Carlos Alberto Labate;
Odalys García Cabrera;
Jorge Mauricio Costa Mondego
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| Advisor: | José Antônio Bressiani; Gonçalo Amarante Guimarães Pereira |
| Abstract | |
Bioethanol is a prominent renewable and green energy source and presents a consistent alternative to the use of fossil fuels. Development of the second generation bioethanol, a new technology to produce this biofuel from plant cell wall, demands the utilization of improved crops with focus on biomass production. Energy cane is a hybrid between Saccharum species selected in breeding programs to present high biomass production and higher content of fiber than sucrose in its composition. To explore the genomics resources and the process of cell wall formation in high biomass hybrids, in this work it was elaborated i) a draft genome of S. spontaneum and ii) the characterization of an elongating energy cane internode. S. spontaneum draft genome contains most of the complete genes of the species, as well as genic expression data of different tissues. The genome comprises a pioneer and important Saccharum database for transcriptomic data assembly and for access of Saccharum sequences of genes (exons and introns) and regulatory regions (promoters). In this study were identified promoter sequences of genes presenting tissue-specific and ubiquitous expression with potential of immediate application in Saccharum biotechnology. Characterization of an energy cane elongating internode demonstrates that it constitutes an excellent model for study of transcriptional control of cell wall formation, especially of vascular bundle lignification. It was observed that the elongating internode could represent the development of the first four immature internodes. Comparative analysis elongating internode of energy cane with internodes from parental genotypes, S. spontaneum and sugarcane, indicated that the elongating internode could explain the differences between hybrids selected for sugar or biomass production. In this project, it was produced a genomic and transcriptomic atlas of S. spontaneum and its high biomass hybrid that will be useful to understand the formation of cell wall in Saccharum (AU) | |
| FAPESP's process: | 12/05890-1 - Saccharum spontaneum genome for development of energy cane |
| Grantee: | Karina Yanagui de Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |