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Metabolic flux analysis applied to the biosynthesis of the biodegradable polymer poly-3-hydroxybutyrate (P3HB) produced by Burkholderia sacchari.

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Author(s):
Débora Vieira Parrine Sant'Ana
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Luiziana Ferreira da Silva; Adalberto Pessoa Junior; Beny Spira
Advisor: Luiziana Ferreira da Silva
Abstract

This work applies Metabolic Flux Analysis to discuss the eficiency of Burkholderia sacchari (LFM101) in PHA production from sugars . Conversion yields of LFM101 and Cupriavidus necator from carbohydrates to PHA were assessed. LFM101 was also grown in reactor fed-batch cultivations, and presented the theoretical maximum in a pseudo-steady stage while grown on glucose. These data were submitted to Metatool and resulted in a metabolic network containing the experimental fluxes of central and PHA metabolism. Cultivation of LFM101 and C. necator under 13C- labeled glucose showed that both species use the same metabolic pathways for the biodegradable polymer synthesis. On a parallel project, the efficiency of biopolymer production from molasses, raw glycerol and acetate in strains producing hydrogen and PHA was tested. Results showed that there was not only an increase in PHA production by R. capsulatus nifH- mutants but also the interaction of light and nitrogen effects were studied by DOE and RSM. (AU)

FAPESP's process: 11/04454-0 - Analysis of metabolic flux applied to the biosynthesis of the biodegradable polymer poly-3-hydroxybutyrate (P3HB) by Burkholderia sacchari.
Grantee:Débora Vieira Parrine Sant'Ana
Support Opportunities: Scholarships in Brazil - Master