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| Author(s): |
Ana Caroline de Souza Barnabé
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2017-12-19 |
| Examining board members: |
Clarice Weis Arns;
João Pessoa Araújo Junior;
Paulo Anselmo Nunes Felippe;
Daniel Fábio Kawano;
Marcelo Lancellotti
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| Advisor: | Clarice Weis Arns |
| Abstract | |
Infections caused by more than one pathogen are common in poultry industry, especially those caused by respiratory agents such as avian metapneumovirus (aMPV) and infectious bronchitis virus (IBV). This study aimed to evaluate a pathology and immune response of specific pathogen free (SPF) chickens coinfected with aMPV and IBV. At 28 days of life, 150 birds were inoculated and divided into 4 groups: negative control, inoculated with IBV, inoculated with aMPV and inoculated with IBV and aMPV. During the experimental period, characteristic clinical signs of infection with the inoculated virus were observed. At 3, 7, 14, 21, 28, and 35 days post inoculation (dpi) the birds were necropsied and blood was collected for serology and flow cytometry, samples of nasal sinus, trachea and lung were collected for viral quantification by real time quantitative PCR (qRT-PCR) and histopathology, and fragments of trachea for evaluation of ciliostasis. Coinfection caused by the two viruses studied caused less damage to birds than the IBV-infected group as observed in flow cytometry, serology and qRT-PCR of the nasal sinus and trachea. In birds inoculated with IBV, both IBV group and aMPV + IBV, there was diarrhea proving with the detection of the virus in the cloacal suabe up to 35 dpi. The evaluation of tracheal ciliostasis did not show large differences between the experimental groups. The lung of the coinfected group had higher numbers of viral copies and larger microscopic lesions in relation to the other groups, differing from the other organs analyzed. The results suggest that simultaneous infection by aMPV and IBV may result in viral interference (AU) | |
| FAPESP's process: | 13/23303-9 - Experimental co-infeccion of infectious bronchitis virus and avian metapneumovirus from autochtounes samples in broilers. |
| Grantee: | Ana Caroline de Souza Barnabé |
| Support Opportunities: | Scholarships in Brazil - Doctorate |