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| Author(s): |
Renata Haddad Pinho
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2020-03-31. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu |
| Defense date: | 2020-02-18 |
| Advisor: | Stelio Pacca Loureiro Luna |
| Abstract | |
Considering the widespread use of rabbits in research that potentially causes pain and discomfort, neglect in the treatment of pain in laboratory animals and the absence of tools capable of adequately assessing pain in this species, the present study aimed to develop and validate a scale of acute postoperative pain in rabbits (RPBS). Footages from three previous studies were used, in which 58 rabbits were submitted to orchiectomy, ovariohysterectomy (OVH) or partial radial ostectomy, recorded at baseline (before surgery); pain (moment of greatest expected pain, after surgery); analgesia (after analgesic rescue - only for animals submitted to orthopedic surgery) and 24h post (24 hours after surgery). The RPBS was developed based on behaviours identified by the ethogram of animals submitted to orthopedic surgery and described in the literature for rabbits undergoing OVH. The behaviours were evaluated by a committee of experts (content evaluation) and then the videos were evaluated by four blinded observers who answered if there was a need for analgesic rescue and scored the proposed scale, as well as the numerical rating (NRS), the simple descriptive (SDS) and the visual analogue scales (VAS). One month after the first evaluation, the videos were again randomized and reassessed. After the evaluations, the scale was refined and validated. According to the principal component analysis, the RPBS was considered unidimensional as it contained adequate eigen-value and variance only in the first dimension. The intra- and inter-observer reliability for the sum of the scale (ICC) was excellent (> 0.80) for all evaluators. According to the Friedman test, the scale was responsive, yet its sum did not reveal any difference between the pain and analgesia moments. By means of the Spearman's correlation, there was a high correlation of RPBS with NRS (0.86), SDS (0.84) and VAS (0.84), and adequate itemtotal correlation (> 0.3). The internal consistency was considered excellent (Cronbach's α coefficient > 0.80). The score ≥3 was the cut-off for rescue analgesia with an area under the curve > 0.90 for all evaluators, demonstrating a high discriminatory capacity of such instrument. Scores 2, 3 and 4 were in the gray zone of diagnostic. Scores were classified as low-intensity pain (1 to 4), intermediate intensity (5 and 6) and high intensity (≥ 7). It was concluded that the proposed scale presented content, criterion and construct validities, responsiveness and reliability to assess acute pain in rabbits. The cut-off for rescue analgesia and the classification of the scores serve as a basis for the administration of analgesics to rabbits submitted to painful procedures. (AU) | |
| FAPESP's process: | 18/17839-7 - Development, validation and reliability of a postoperative acute pain scale in rabbits (Oryctolagus cuniculus) |
| Grantee: | Renata Haddad Pinho |
| Support Opportunities: | Scholarships in Brazil - Master |