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| Author(s): |
João Paulo Fabi
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2007-08-24 |
| Examining board members: |
João Roberto Oliveira do Nascimento;
Beatriz Rosana Cordenunsi;
Marília Gaspar;
Carlos Alberto Labate;
Eduardo Purgatto
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| Advisor: | João Roberto Oliveira do Nascimento |
| Abstract | |
Ripening affects the quality of fleshy fruits by changing the chemical composition, and gene expression regulates most part of these transformations. Although papaya fruit is an important Brazilian crop, there are restricted studies concerning its ripening and gene expression. In this work, it is described the use of some RNA profiling techniques, such as Differential Display-PCR, cDNA-AFLP and Cross-Species Microarray, to identify ripening-related genes in papaya fruit. Using these techniques, some differentially expressed genes during papaya fruit ripening were isolated. Their functions were associated with metabolism and transport of sugars, lipids and carotenoids, cell wall disassembly, stress and transcription factors dependent and independent of plant hormones. In this way, it was proposed a pattern of gene expression during papaya fruit ripening, at least for those isolated genes, and the assemblage of these data with other authors data can locate fruit handling to a better level, minimizing postharvest losses and increasing papaya fruit shelf-life. (AU) | |