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| Author(s): |
Alex Issamu Kanno
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-10-18 |
| Examining board members: |
Luciana Cezar de Cerqueira Leite;
Jose Alexandre Marzagao Barbuto;
Sylvia Luisa Pincherle Cardoso Leão;
Gabriel Padilla Maldonado;
Ricardo De Marco
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| Advisor: | Luciana Cezar de Cerqueira Leite |
| Abstract | |
BCG is an attenuated bacillus derived from Mycobacterium bovis currently being investigated to express foreign antigens. Genes from viruses, bacteria and parasites are cloned to mycobacterial vectors and with them, recombinant BCG strains are created. Recombinant BCG and M. smegmatis strains where capable to express SmStoLP-2 and mice immunized with these rBCGs do not show better protection against infection with cercariae, although the distinct immune response observed. The use of codon optimization made possible the expression of SmTSP-2 and SmVAL-5 in M. smegmatis but not BCG. M. smegmatis transformed with a library of plasmids containing the L5p upstream egfp gene show a wide range in fluorescence. 3 of these plasmids strong and weak defined as their ability to produce GFP were used to express in BCG the antigen Sm29 at different levels by each promoter. (AU) | |
| FAPESP's process: | 08/04631-7 - Recombinant BCG expressing Schistosoma mansoni surface antigens: immune response and protection assessment |
| Grantee: | Alex Issamu Kanno |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |