Development of enzymatic amperometric biosensor for the determination of clinical importancy compounds

Author(s)

Pablo Alejandro Fiorito

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ)
• Defense date: 2005-11-29

Examining board members

Susana Ines Cordoba de Torresi; Orlando Fatibello Filho; Renato Sanches Freire; Lauro Tatsuo Kubota; Hideko Yamanaka

• Advisor: Susana Ines Cordoba de Torresi

Abstract

This work describes the preparation and characterization of modified electrodes with Prussian blue and some analogues and their application in the development of amperometric enzymatic biosensors for the detection of glucose and oxalate. The materials used in electrode modification were Prussian blue and hybrid compounds, formed by nickel hexacyanoferrate and polypyrrole or copper hexacyanoferrate and polypyrrole. These materials were able to mediate the hydrogen peroxide electroreduction even in electrolytes containing Na+, showing better analytical performance than the hexacyanyoferrates without polypyrrole. These materials were successfully used to build up oxalate and glucose biosensors. We also have studied the preparation of glucose biosensors using the layer by layer self assemble technique (LBL), which has optimized the enzyme immobilization process, obtaining good analytical performances even loading small amounts of enzyme. FinaIly, we have described the synthesis and characterization of Prussian blue nanoparticles by a sonochemical method. It was possible to synthesize nanoparticles with a diameter around 5 nrn, which were immobilized by the LBL technique, in order to study their electrochemical behavior. (AU)