Evaluation of immunomodulation induced by crotoxin from Crotalus durissus terrificus snake venom in experimental model of inflammatory bowel disease.

Author(s)

Caroline de Souza Almeida

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-06-16

Examining board members

Eliana Faquim de Lima Mauro; Ana Paula Ferreira; Marcelo de Franco; Dunia Del Carmen Rodriguez; Sandra Coccuzzo Sampaio Vessoni

• Advisor: Eliana Faquim de Lima Mauro

Abstract

In this work it was analyzed the immunomodulatory effect of crotoxin (CTX) isolated from C.d. terrificus snake venom, on the experimental model of colitis induced by TNBS in mice. The CTX was able to inhibit the weight loss, clinical and histological score, MPO synthesis and pro-inflammatory cytokines. Lower number of neutrophils and macrophage (M1 and M2) in lamina propria was observed in TNBS/CTX mice compared with the TNBS group. In contrast, the CTX induced increased TGF-b, IL-10, PGE2 and LXA4. The in vivo neutralization of these cytokines or eicosanoids synthesis indicates that these molecules exert significant role in the modulatory effect of CTX. The analyzes of distinct cell populations from lamina propria, lymph nodes and Peyers pathes showed no difference in CD4+Tbet+ between TNBS or TNBS/CTX mice. However, CTX induced an increase of CD4+FoxP3+ and decreased CD4+RORgt+. Together, these results indicate that CTX is able to modulate intestinal acute inflammatory response induced by TNBS improving the clinical status of the mice. (AU)

• FAPESP's process: 10/05701-9 - Evaluation of imunnomodulation induced by crotoxin isolated from Crotalus durissus terrificus in experimental model of inflammatory bowel disease
• Grantee: Caroline de Souza Almeida
• Support Opportunities: Scholarships in Brazil - Doctorate