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| Author(s): |
Fabio Sergio Cury
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2016-12-19 |
| Examining board members: |
Carlos Eduardo Ambrosio;
Claudio Gustavo Barbeito;
Celina Almeida Furlanetto Mançanares;
Moacir Franco de Oliveira;
Felipe Perecin
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| Advisor: | Carlos Eduardo Ambrosio |
| Abstract | |
The preservation of wild Brazilian species is based on knowledge, distribution, behavior both in the natural environment and in captivity, therefore, there is a great importance in the case of reproductive description of the species studied always aiming the perpetuation. This study has focused on the uterine detailing and their reproductive whole, related to placentation process, in addition to evolution as the phylogenetic position of mammals, describing the morphology of the placenta, placental barrier in delimitation of phylogenetic evolution of this organ in animals of the species Coendou prehensilis. The C. prehensilis, popularly called porcupine clerk or porcupine, is an herbivorous rodent that usually lives in tropical forest trees from Mexico to South America, is a mammal that can weigh 2-5 pounds and measure 30 to 60 centímeters. For the study, 07 animals (2 pregnant and 5 non-pregnant) were used, all animals died by dog attacks and trampling on roads, the research was regularized by IBAMA. Macroscopic techniques were used describing the format, vascularization and biometry of the reproductive organs and placenta of the species in question, besides light microscopy, immunohistochemistry (vimentin 1: 200, PCNA 1: 700 and cytokeratin 1: 400), electron microscopy scanning and transmission electron microscopy to characterize the placental barrier and to describe maternal-fetal relations in the placenta. The ovaries are ellipsoid in shape, yellow in color and are flattened dorsi-ventrally and are relatively large, with smooth wall variations presenting different follicular phases. The uterine tubes are tubular, cylindrical and tortuous. They extend laterally, curving craniocaudally on the ovaries. They are not continuous in thickness and shape, being possible to distinguish infundibulum, ampule and isthmus. The uterus is bicornuous in a \"Y\" format with two long cylindrical horns, along its lumen does not present a median septum. The vagina has non-keratinized squamous stratified epithelium, with longitudinal folds, the number of folds is variable as well as its thickness. The vulva has a difference in tegumentary color of the big lips in relation to the rhyme. The clitoris is located in the ventral portion of the rhyme behind the upper labial commissure and is not visible. The placenta is chorioallantoic, zoonary, discoid, labyrinthine type of lobular pattern, corresponding to the main region for maternal-fetal exchanges. And fetal vessels were identified by vimentin - positive endothelium, in contrast to the maternal blood spaces. Giant sinusoidal cells were identified in the labyrinth and close to decidua. The visceral yolk sac is composed of cubic cells of the endoderm, a layer of mesenchyme rich in pots and mesothelium. It has a subplacenta and presents a maternal-fetal relationship of the hemomonochorial subtype, besides a yolk sac present until the end of gestation. With the results of the structural analysis of the material, we hope to contribute to improve the data on the reproductive aspects of the species, collaborating in other future research, and generating a comparative base for similar South American mammals in our territory. (AU) | |
| FAPESP's process: | 13/05839-9 - Placental development and morphology of the female reprocuctive organ in Coendou prehensilis(Pocupine Clerk) |
| Grantee: | Fabio Sergio Cury |
| Support Opportunities: | Scholarships in Brazil - Doctorate |