Investigation of genes differentialy expressed in intra-host stages of Schistosoma mansoni as vaccine candidates.

Author(s)

Cibele Aparecida Tararam

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-04-20

Examining board members

Luciana Cezar de Cerqueira Leite; William de Castro Borges; Luis Carlos de Souza Ferreira; Ricardo De Marco; Glaucia Maria Machado Santelli

• Advisor: Luciana Cezar de Cerqueira Leite

Abstract

Schistosomiasis is an important disease in public health. Genes selected from the S. mansoni transcriptome, 56% of them were confirmed as differentially expressed in schistosomula by real time RT-PCR. Among them, the protein Ly6.5 is present in the tegument of schistosomula and adult worms by GPI anchors. The function of inhibiting the complement system was not detected, but it may be involved in maintenance of the tegument. The gene SmVal7 revealed transcripts in the esophageal glands of adult worms by in situ hybridization, while the localization of the protein is not defined. Annexin is associated with the membranes of the schistosomula and adult worms tegument in a calcium-dependent manner. The suppression of the gene by RNAi did not resulted in a significant phenotypic change in schistosomula in vitro. Parcial inhibition of the coagulation activity and potential function of endocytosis of membrane-bound antibodies were observed. Immunization with the rLy6.5, rSmVal7, rAneI or rAneII-II-III did not show reduction in worm burden recovery after challenge. (AU)

• FAPESP's process: 05/03781-7 - Investigation of genes differentially expressed in intra-host stages of Schistosoma mansoni as vaccine candidates
• Grantee: Cibele Aparecida Tararam
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)