Cosmetic formulations containing Pothomorphe umbellata root extract to prevent pho...
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Prevention of photo-oxidative damage to the skin for topical treatment with root extract of Phothomorphe umbellata L. Miq
Full text
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| Author(s): |
Cristina Dislich Ropke
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2003-05-29 |
| Examining board members: |
Silvia Berlanga de Moraes Barros;
Elfriede Marianne Bacchi;
Maria Vitoria Lopes Badra Bentley;
Paolo Di Mascio;
Valéria Petri
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| Advisor: | Silvia Berlanga de Moraes Barros |
| Abstract | |
Topical administration of antioxidants provides an efficient way to enrich the endogenous antioxidant system and thus may be a successful strategy for diminishing ultraviolet radiation-mediated oxidative damage in the skin. The Brazilian Flora is rich in medicinal plants with a high potential to provide these antioxidant substances. Crude dried root ethanolic extracts of Pothomorphe umbellata, from the piperaceae family, demonstrated a significant activity in the prevention of in vitro spontaneous brain lipid peroxidation. This activity was attributed to 4-nerolidylcathecol, a compound isolated from the hexane extracts of roots and leaves of P. umbellata. In this work we evaluated the influence of topical application of P. umbellata root extract gel, containing 0,1% of 4-nerolidylcathecol, on the antioxidant network in ultraviolet-induced oxidative damage in hairless mouse skin. In this study we evaluated the influence of three different formulations (gel, gel-emulsion and emulsion) on the percutaneous absorption of 4-nerolidylcathecol, an antioxidant compound isolated from Pothomorphe umbellata root extracts. Also the absorption of the isolated 4-nerolidylcathecol was compared with its absorption when dried Pothomorphe umbellata root ethanolic extract was incorporated in gel and gel-emulsion formulations. The 4-nerolidylcathecolgel formulation presented the higher rate of penetration, leading to higher dry drug levels in the tissue.4-nerolidylcathecol was also absorbed in biological conditions and was stable when exposed to UV-irradiation. The UV-irradiation had no influence on ascorbic acid levels, or on the antioxidant enzymes activities, but topical P. umbellata treatment protected α-tocopherol from depletion after UV-irradiation. After UV-irradiation α-tocopherol concentration decreased significantly (≈40%, p<0.01) in irradiated control groups, while in the P. umbellata treated group, a-tocopherol was totally preserved (≈100%, p>0.05). We also tested the extract activity on animals chronically exposed to UV-radiation. The extract was able to prevent photoaging of the irradiated animals skin. These data demonstrate that P. umbellata may successfully employed as a topical photoprotective agent. (AU) | |
| FAPESP's process: | 99/03074-6 - Cosmetic formulations containing Pothomorphe umbellata root extract to prevent photo-oxidative skin damage |
| Grantee: | Cristina Dislich Ropke |
| Support Opportunities: | Scholarships in Brazil - Doctorate |