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| Author(s): |
Vanessa Aparecida Gonçalves Giozzet
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2008-11-02 |
| Examining board members: |
José Roberto Bosqueiro;
Silvana Bordin;
Eliana Pereira de Araujo;
Angelo Rafael Carpinelli;
Marcio Alberto Torsoni
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| Advisor: | José Roberto Bosqueiro |
| Abstract | |
Malnutrition caused by protein restriction and dexamethasone -induced insulin resistance, in vivo treatment (Dex) are conditions associated with morphological and functional alterations in pancreatic islets. Thus, the present study evaluated the dexamethasone treatment effects on the metabolic parameters, glucose-stimulated insulin secretion and proteins involved in the insulin - signalling pathway over low protein diet fed rats (LP). LP rats showed decrease in body weight, serum insulin, total serum protein, and serum albumin, patte rns that characterize the LP rats. Moreover, LP rats presented improved peripheral insulin sensibility and reduced islets area (P < 0,05). Except for the body weight (P < 0,05), all these parameters were proned to be normalized in rats exposed to a low protein diet and treated with dexamethasone (LPD), whose islets showed increased glucose stimulated insulin secretion (GSIS). In addition, LPD rats showed lower protein expression of IRS-1, IRS-2 and higher in p-FoxO1, p-ERK and PKC, while presenting pancreatic islet hypertrophy compared to LP rats islet. In conclusion, dexamethasone treatment revert the effects related to metabolism and islet function caused by diet protein restriction, confirming ß-cells wide plasticity, even in transient or lasting adverse conditions (AU) | |