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| Author(s): |
Natalie Michele de Souza
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-04-25 |
| Examining board members: |
Ana Lucia Tabet Oller do Nascimento;
José Gregorio Cabrera Gomez;
Maria Regina D'Imperio Lima
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| Advisor: | Ana Lucia Tabet Oller do Nascimento |
| Abstract | |
Leptospirosis is a zoonotic disease caused by pathogenic bacteria of genus Leptospira. In the world, nearly 500,000 cases are reported each year, with 10% of mortality rate. Currently, vaccines against leptospirosis are composed by inactivated cells that are ineffective in many aspects. After genome analysis, the genes LIC11121, LIC11087, LIC11228 e LIC11084 were chosen for immunogenicity characterization of their respective proteins. These genes were cloned in the pAE expression vector and the proteins encoded by LIC11087, LIC11228 and LIC11084 were purified. The results suggest the localization of these proteins in the bacterial outer membrane, are immunogenic, are possibly expressed during infection and may have involvement in mechanisms of immune system evasion and pathogenicity. Moreover, in one of two experiments, the rLIC11084 protein induced partial protective immunity of immunized hamsters against lethal challenge. (AU) | |
| FAPESP's process: | 11/02137-8 - Immunogenic activity evaluation of two predicted membrane proteins of Leptospira interrogans expressed in Escherichia coli |
| Grantee: | Natalie Michele de Souza |
| Support Opportunities: | Scholarships in Brazil - Master |