Differential proteomic analysis of mango fruit pulp (Mangifera indica L.) during postharvest ripening

Author(s)

Jonathan de Magalhães Andrade

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ)
• Defense date: 2011-05-16

Examining board members

João Roberto Oliveira do Nascimento; Marília Gaspar; Franco Maria Lajolo

• Advisor: João Roberto Oliveira do Nascimento

Abstract

Mango fruit (Mangifera indica L.) is cultivated in tropical and subtropical areas, mainly in developing countries. India, China, Mexico, Indonesia, Thailand, Pakistan and Brazil are the major producers, but its trade has been limited due the highly perishable nature of the fruit. During ripening, the fruits acquire characteristics that make them appropriate for consumption as a consequence of metabolic changes dependent on the expression of specific genes. As proteins are the effector elements of gene expression, proteome analysis can help the identification of metabolism keypoints that could influence the fruit quality. Thus, the aim of this study was to compare the protein maps of mango pulp (Mangifera indica L. cv. Keitt) in pre-climacteric and climacteric stages, in order to identify protein spots that differ in abundance in these two stages. After protein extraction and separation by 2D-DIGE technique, the gel images obtained were analyzed with PDQuest software, using the Student´s T-test for statistical analysis. We obtained 47 spots differently abundant between the stages studied, that were excised from the gels and its proteins digested with trypsin and sequenced by mass spectrometry. We obtained the identities of 58 distinct proteins from the search of the peptide sequences against NCBI2010 database using the software Mascot. (AU)

• FAPESP's process: 09/02658-8 - Differential analysis of the mango fruit proteome during ripening
• Grantee: Jonathan de Magalhães Andrade
• Support Opportunities: Scholarships in Brazil - Master