Molecular and morphological characterization of Aedes aegypti populations (Diptera: Culicidae) from State of São Paulo.

Author(s)

Paloma Oliveira Vidal

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-11-17

Examining board members

Lincoln Suesdek Rocha; Andréa Cristina Fogaça; Paulo Eduardo Martins Ribolla; Maria Anice Mureb Sallum; Denise Selivon Scheepmaker

• Advisor: Lincoln Suesdek Rocha

Abstract

The State of São Paulo displays one of the highest rates of dengue infection in the world, but despite this fact, a few populational studies of Ae. aegypti have been undertaken. The aim of this study was to genetically and morphologically characterize Ae. aegypti populations from six locations in the São Paulo State (Santos, S.P., Campinas, São Carlos, Catanduva, S.J.R.P.) during 2011 and 2012. The phenetic and genetic analyses revealed that populations of Ae. aegypti are structured. Eight microsatellites loci were polymorphic and genetic differentiation among samples was moderate (Fst= 0.04; p < 0.05). Nucleotide diversities of COI (&pi; = 0.0062) and ND4 gene (&pi; = 0.017) were moderately high. Two lineages distinct genetically were found in the State. Over the months comprised by the study, we found the temporal genetics and morphologics differences among the six populations, a possibly indicative of microevolution of mosquitoes. The results of this study may be useful for understand the spread of this vector mosquitoes in the State of São Paulo. (AU)

• FAPESP's process: 10/15039-1 - Molecular and morphological characterization of Aedes aegypti populations (Diptera: Culicidae) from State of São Paulo
• Grantee: Paloma Oliveira Vidal
• Support Opportunities: Scholarships in Brazil - Doctorate