Design, synthesis and evaluation of antimicrobial activity of novel furfuryliden derivatives against nosocomial infections

Author(s)

Rodrigo Rocha Zorzi

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ)
• Defense date: 2013-12-17

Examining board members

Leoberto Costa Tavares; João Paulo dos Santos Fernandes; Kerly Fernanda Mesquita Pasqualoto

• Advisor: Leoberto Costa Tavares

Abstract

Nosocomial infections are caused by bacteria and fungi and are the main reason for thousands of deaths every year in the hospital environment. The most susceptible individuals to these type of infection are the immunocompromised, highlighting old-aged and immunodeficiency people, as also patients who passed by invasive procedures. Not only do this situation is very serious, but also a huge number of multidrug-resistant microorganisms are reported worldwide, basically due to excess of prescription and wrong use of antibiotics. If this situation doesn\'t be maintained today, there will not be a bright sight in the future. In this context, there is the necessity for research and development of new antimicrobial agents, and the nitrocompounds, highlighting nifuroxazide\'s analogs, are showing excellent activity against several pathogens related to multidrug resistant nosocomial infections. Therefore, this work aims to design, to synthesize and to evaluate two series of nifuroxazide\'s analogs against strains of gram-positive and gram-negative bacteria, also fungi strains. The sets of analogs were designed based on the replacement of hydroxyl group of nifuroxazide by different substituent groups according to Craig\'s diagram, also a modification in the structure core; identification of these compounds was carried out through 1H and 13C NMR. Melting point and elementary analysis were analyzed for purity criteria. Until the moment, about 23 compounds were evaluated and the best observed activity was against S. aureus strains, which 16 analogs showed better activity than the lead compound and several other compounds, used as referential drug. It is noteworthy that the best activity were analogs 4-butyl-N\'-((5-nitrofuran-2-yl) methylene) benzohydrazide and 3-acetyl-2-[5-nitro-furan-2-yl]-5-[4-cyano-phenil]-2,3-dihydro-1,3,4 oxadiazole with IC90 = 1,8 ± 0,04 µM and 3,89 ± 0,07 µM, respectively. The chemometrics studies suggest that electronic properties are most related to the biological activity on these microorganisms. All the available results shows the potential of nitrofurans to be a new candidate for an antimicrobial drug against multidrug resistant nosocomial infections. (AU)

• FAPESP's process: 11/14773-6 - Design, synthesis and evaluation of antimicrobial activity of novel furfuryliden derivatives against nosocomial infections.
• Grantee: Rodrigo Rocha Zorzi
• Support Opportunities: Scholarships in Brazil - Master