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| Author(s): |
Vinicius André Morais Rocha Melo
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2009-04-29 |
| Examining board members: |
Bryan Eric Strauss;
Vagner Roberto Antunes;
Eliane Namie Miyaji
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| Advisor: | Bryan Eric Strauss |
| Abstract | |
Formation of tumors it must to combinations of factors. The p53 pathway has an essential role in proliferation control and apoptosis. The interferon-beta (IFNb) is important in modulation of the immunologic response, in the antitumoral effect and in the apoptotic impact in tumor cells. According to literature, IFNb activate the p53 transcription and components of IFN system effect its function to p53/p14arf pathway. In this project, a series of tools was constructed to explore interactions between p53 and IFNb. The first tool, a cellular lineage derivative of B16 with expression of p53 reduced by miRNA. We also construct plasmidial and adenoviral vectors carriers of cDNAs for eGFP, Luciferase, p53 or IFNb. The vectors are used to introduce these factors, alone or agreed, in the target cell. Even confirming the activity of p53 or IFNb alone, an additive effect of these factors combined was not observed with this type of assay. Future studies of the possible interactions between p53 and IFNb pathways will have the benefit of the tools constructed in this project. (AU) | |
| FAPESP's process: | 05/03625-5 - p53 role in interferon beta antitumor effect evaluated in a melanoma model |
| Grantee: | Vinicius André Morais Rocha Melo |
| Support Opportunities: | Scholarships in Brazil - Master |