Full text
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| Author(s): |
Lorena do Nascimento Pantaleão
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2013-01-22 |
| Examining board members: |
Maurício Yonamine;
Karina Helena Morais Cardozo;
Ernani Pinto Junior
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| Advisor: | Maurício Yonamine |
| Abstract | |
Amphetamines are a class of compounds with chemical structures similar to phenylethylamines that present stimulant activity in the central nervous system. Anorectic drugs such as fenproporex and diethylpropion and some illicit drugs as methamphetamine (ice, speed) and metilenodioximetamphetamine (MDMA, ecstasy) are also included in this class. These substances have a high abuse potential, and they can be used by various social groups, for example, professional drivers (who call them \"rebites\"), young students (using ecstasy in \"rave\" parties) and people who abuses anorectics to weight control. Interestingly, there is also a possibility of some people using anorectics and benzodiazepines in association. Although the commercialization of amphetamine derivatives has been recently banned in Brazil (2011), it is known that some people still use anorectic drugs and benzodiazepines to control insomnia induced by amphetamines. Another case of concomitant use would be professional drivers, who use \"rebites\", which also try to control their sleep cycles using benzodiazepines. Because of this situation, it would be interesting to monitoring the use of these drugs by toxicological analysis. The conventional toxicological analyses (performed in most cases in blood and urine) generally provide small window detection. In this case, intermittent drug use may not be detected. When long term information about drug use is needed, the most efficient matrix to carry out the analysis is hair. In this project, analytical methods were developed for the determination of amphetamines (amphetamine, methamphetamine, MDMA, MDA and fenproporex) and benzodiazepines (diazepam, nordiazepam, chlordiazepoxide, oxazepam, clonazepam and temazepam) in hair samples. Liquid-phase microextraction (LPME) and solid phase extraction (SPE) were used as sample preparation techniques in these methods. Analites were identified by gas chromatography/mass spectrometry (GC-MS). After the development, optimization and validation, the methods were applied in hair samples collected from patients of a drug rehabilitation clinic. The results obtained with the application of the developed methods showed their efficacy for the intended purpose. (AU) | |
| FAPESP's process: | 10/03342-1 - Simultaneous toxicological analysis of amphetamines and benzodiazepines in hair samples |
| Grantee: | Lorena Do Nascimento Pantaleão |
| Support Opportunities: | Scholarships in Brazil - Master |